Agilent Lab-on-a-Chip RNA Quality Check
The Agilent Lab-on-a-Chip assay leverages microfluidics technology, enabling the quality analysis of RNA using only 1ul of sample. The assay is run on the Agilent 2100 Bioanalyzer instrument and utilises the Agilent 2100 Expert Software to analyze and display results. An RNA Integrity Number (RIN score) is generated for each sample on a scale of 1-10 (1=lowest; 10=highest) as an indication of RNA quality. The 18s/28s ratio and an estimation of concentration is also produced.
| Chip Type | RNA type |
Concentration Range |
max # of samples per chip |
|---|---|---|---|
| RNA Nano Chip | total RNA |
25-1000ng/ul |
12 |
| RNA Nano Chip | mRNA |
25-500ng/ul |
12 |
| RNA Pico Chip | totalRNA |
0.2-5ng/ul |
11 |
| RNA Pico Chip | mRNA |
0.5-5ng/ul |
11 |
Articles Related to RNA Quality Check with Agilent Bioanalyzer
- RNA Quality.ppt
- Using RIN.pdf
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- mRNA with Agielnt Bioanalyzer
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- Whole blood and leukocyte isolation.pdf
- Whole Blood Sample Prep for gene expression microarray.ppt
- Globin reduction protocol A method.pdf
- Quantitation with agilent bioanalyzer ribogreen UV.pdf